Accessing the features of surface neuraminidase (N1) of influenza A virus presenting on the platforms for anti-NA Abs screening
10.3760/cma.j.issn.1003-9279.2013.01.001
- VernacularTitle:对流感病毒神经氨酸酶N1亚型抗体筛选体系的评价
- Author:
Lan HUANG
1
;
Kun QIN
;
Jian-Fang ZHOU
;
Yue-Long SHU
;
Hong WEI
Author Information
1. 400014,重庆医科大学附属儿童医院新生儿科
- Keywords:
Neuramindase;
Eukayotic cells;
Expressed sequence tags;
Yeasts;
Antibody,viral
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand if the Neuraminidase (N1) of Influenza A virus at the surface of yeast-displaying system,eukaryotic expression system and the infected cells could be used for anti-NA Abs screening,their activities and bindings to five candidate Abs were assayed.Methods The surface NA expression was obtained by transfecting by recombinant NA constructors with specific tag-labels or live virus infection.The functional activity was measured by the fluorescent assay.Their bindings to the Abs were detected by flow cytometry.Results The surface NAs presenting on the yeast-displaying system and eukaryotic expression system exhibited functional NA activities as the NA at the surface of virus-infected cells which showed affinities to Ab1,4,and 5.The same bindings to Ab1 and 5 were found in the surface NA expressed by eukaryotic expression system while minor binding was observed in the yeast displayed-NA.Conclusion The epitopes of yeast-displayed NA may be different from the NAs present at eukaryotic expression system and the infected cells which more likely suitable for the screening of anti-NA Abs.
