Analysis the viral etiology of fever and respiratory tract infection syndrome in Shaanxi province during 2010
10.3760/cma.j.issn.1003-9279.2013.01.003
- VernacularTitle:陕西省发热呼吸道症候群病原谱和呼吸道合胞病毒基因特征
- Keywords:
Respiratory tract infections;
Respiratory syncytial viruses;
Epidemiology,molecular
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(1):8-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective Analysis the viral pathogenic spectrum for patients with fever and respiratory tract infection syndrome in Shaanxi province during 2010 and investigate the molecular epidemiology characteristics of respiratory syncytial virus.Methods A total of 208 patients' pharyngeal swabs were collected based on surveillance definition from January 2010 to January 2011 and screened for sixteen human respiratory virus types/subtypes by Qiaxcel-based multiplex reverse transcription-PCR assay,including HRV,HCoV,Flu,HPIV,ADV,HRSV,HMPV and HBoV and investigate molecular epidemiology of HRSV by sequencing and phylogenetic analysis of the C-terminal second hypervariable region of the G gene.Results 109 out of 208 specimens (53％) were positive for one or more viruses.HRSV(42.2％) was the dominant pathogen detected,followed by Flu(24.5％),PIV(20％),HRV(13.6％) and ADV(10.9％),there were also 8 strains of HCoV,5 strains of HMPV and 3 strains of HBoV detected.The results showed that 22specimens were positive for two or more viruses,PIV (14/22) was the most frequently detected viral agent among co-infection specimens,and the highest incidence of mixed infection is aged 15-39 years group (P ＜0.05).The overall viral detection rate was no related to age.In addition to Flu,HMPV and PIV,other viruses (HRV,HBoV,HCoV,ADV,RSV) mainly infected 0 to 4 years old children.Among 46 HRSV positive specimens,42 HRSV-A strains clustered into NAI genotype and two HRSV-B strains clustered into two genotypes,BA9 and GB2.Conclusion HRSV is the dominate pathogen collected from patients with fever and respiratory tract infection syndrome in Shaanxi and HRSV A is the predominant subtype.For most viruses,infection was most prevalent among children aged ＜ 4 years.PIV was the most common pathogen in co-infection.
