Study on BALB/c mice with EV71 infection by diverse inoculation routes
10.3760/cma.j.issn.1003-9279.2013.01.005
- VernacularTitle:EV71不同接种途径对BALB/c小鼠的感染特点
- Author:
Peng LI
1
;
Ying-Ying YUE
;
Nan-Nan SONG
;
Zhi-Hui LI
;
Hong MENG
Author Information
1. 250062 济南,山东省医学科学院基础医学研究所,山东省罕少见病重点实验室
- Keywords:
Enterovirus;
Mice,inbred BALB/c;
Model,animal
- From:
Chinese Journal of Experimental and Clinical Virology
2013;27(1):14-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the characteristics of EV71 JN200804 strain infection in one-day old BALB/c mice and to establish a animal model of EV71 infection,and to provide information and technical support for the evaluation of the EV71 vaccine and antiviral medicine.Methods One-d/ay old BALB/c mice were infected with EV71 JN200804 strain through oral(PO),intracranial(IC),intraperitoneal (IP),intramuscular (IM) routes,respectively.All mice were sacrificed at paralysis of hind limbs and collected organs for viral isolation,RT-PCR and pathological examination,and the electrophysiology were detected before sacrifice.Results All mice infected through IC,IP and IM routes were paralyzed in hind limbs at 4-5 days and died at 7 days about,the hypokinesia and lethargy of mice were observed through PO routes.The viruses could be isolated and detected in the muscle from mice infected through IC,IP and IM routes and in the spinal cord through IC routes by viral isolation and RT-PCR.The neurogenic and myogenic disorders were detected by electromyography.Histopathologically,the varied pathologic changes were observed in the mouse cerebellum,spinal cord,muscle,heart,lung,liver and kidney.Conclusion EV71 JN200804 strain can infect one-day old BALB/c mice and induce paralysis of hind limbs,its animal infection model may apply to study of EV71 infection pathogenesis and antiviral medicine,and evaluation of the EV71 vaccine.
