Relationship between aging and the number and function of bone marrow-derived endothelial progenitor cells in rats.
- Author:
Jian-fei CHEN
1
;
Lan HUANG
;
Jun JIN
;
Xiao-jing WU
;
Lan LIU
;
Jian ZHOU
;
Xiao-hui ZHAO
;
Bin CUI
Author Information
- Publication Type:Journal Article
- MeSH: Age Factors; Animals; Bone Marrow Cells; cytology; Cell Count; Cells, Cultured; Endothelial Cells; cytology; Monocytes; physiology; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Cardiology 2006;34(11):1026-1028
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between aging and the number and function of bone marrow-derived endothelial progenitor cells (EPC) in rats.
METHODSThe number and function of bone marrow-derived EPC were measured in 4, 10 and 18 months old rats (n = 10 in each group). Mononuclear cells (MNC) were isolated from rat bone marrow by Ficoll density gradient centrifugation and cultured for 7 days, EPC were identified as adherent cells double positive stained for FITC-UEA-I and DiI-acLDL under laser confocal immunofluence microscopy. Boyden chamber was used to determine the migration capacity of EPC and the number of recultured EPC was counted to test the adherent function of EPC.
RESULTThe number (42.0 +/- 5.8 vs 30.8 +/- 4.7 vs 21.5 +/- 4.7 per field) and the migration and adhesion capacities of EPC were significantly reduced with aging.
CONCLUSIONThis study shows that the number and function of EPC are decreased with aging.
