The effects of various beta-blockers on myocardial gap junction structure in rat with myocardial ischemia-reperfusion injury.
- Author:
Shu-ying FAN
1
;
Yuan-nan KE
;
Yu-jie ZENG
;
De-chun SU
;
Yong WANG
;
Pei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adrenergic beta-Antagonists; pharmacology; Animals; Connexin 43; metabolism; Disease Models, Animal; Gap Junctions; drug effects; Male; Myocardial Reperfusion Injury; Myocardium; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Cardiology 2007;35(2):182-186
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the effects of carvedilol, metoprolol and propranolol on myocardial gap junction (GJ) structure in rat with myocardial ischemia and reperfusion injury.
METHODSRats were divided randomly into five groups: sham operation group (SO), myocardial ischemia and reperfusion group (IR), IR + carvedilol group (CV), IR + metoprolol group (MT), and IR + propranolol group (PP). The left anterior descending branch was ligated for 30 minutes and reperfused for 4 hours (IR). After 4 h reperfusion, the distribution and composition of gap junctional connexin 43 (CX43) were observed by immunofluorescence and laser scanning confocal microscopy (LSCM), and the quantification of CX43 was measured by LSCM.
RESULTCompared with SO group, IR resulted in abnormal distribution and composition of CX43-GJ and the impairment of CX43-GJ was significantly attenuated by CV, MT and PP treatments with the best effect observed in CV group (P<0.05 vs. MT and PP).
CONCLUSIONThese results suggest that beta-blockers, especially, carvedilol, could significantly attenuate IR induced CX43-GJ impairment.
