The effects of Tongxinluo Supermicro Powder on nuclear factor-kappaB, intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression in aorta of rabbits fed with high-lipid diet.
- Author:
Yi-ling WU
1
;
Jia-hua YOU
;
Guo-qiang YUAN
;
Jun-qing LIANG
;
Zhen-hua JIA
;
Ke-jian LIU
;
Cong WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animal Feed; Animals; Aorta; drug effects; metabolism; Dietary Fats; adverse effects; Drugs, Chinese Herbal; pharmacology; Intercellular Adhesion Molecule-1; metabolism; Male; NF-kappa B; metabolism; Rabbits; Vascular Cell Adhesion Molecule-1; metabolism
- From: Chinese Journal of Cardiology 2007;35(3):271-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of Tongxinluo Supermicro Powder on the nuclear factor-kappaB (NF-kappaB), inter-cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in aorta of rabbits fed with high-lipid diet.
METHODSHealthy male New Zealand rabbits were randomly divided into 4 groups (n = 8 each): control group, model group, atorvastatin group (3 mg x kg(-1) x d(-1) per gavage), and Tongxinluo group (0.31 g x kg(-1) x d(-1) per gavage). At the end of 6 weeks, the expression of NF-kappaB, ICAM-1 and VCAM-1 were observed by immunochemistry methods, Western blotting and reverse transcription polymerase chain reaction (RT-PCR).
RESULTThe nuclear translocation of NF-kappaB in aortic endothelial cells and the gene expressions of NF-kappaB, ICAM-1 and VCAM-1 at protein and mRNA levels of the model group was significantly increased compared that in the control group (all P < 0.05), these effects could be significantly attenuated by atorvastatin and Tongxinluo Supermicro Powder (P < 0.01 vs. model group).
CONCLUSIONSSimilar as atorvastatin, Tongxinluo Supermicro Powder could relieve the process of atherosclerosis by decreasing the nuclear translocation of NF-kappaB and reducing the expression of ICAM-1, VCAM-1 in this model.