Autologous bone marrow mononuclear cells and peripheral endothelial progenitor cells differentiation in myocardial ischemia reperfusion injury region in swine.
- Author:
Chong-Jian LI
1
;
Run-Lin GAO
;
Yue-Jin YANG
;
Lai-Feng SONG
;
Ying-Mao RUAN
;
Feng-Huan HU
;
Wei-Xian YANG
;
Ji-Lin CHEN
;
Shu-Bin QIAO
;
Xue-Wen QIN
;
Yu-Qing LIU
;
Zai-Jia CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; Cell Differentiation; Cell Survival; Cells, Cultured; Disease Models, Animal; Endothelial Cells; cytology; transplantation; Mesenchymal Stem Cell Transplantation; Monocytes; transplantation; Myocardial Reperfusion Injury; blood; Stem Cells; cytology; Swine; Swine, Miniature; Transplantation, Autologous
- From: Chinese Journal of Cardiology 2007;35(4):350-353
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differentiation status of autologous bone marrow mononuclear cells (BM-MNC) and peripheral endothelial progenitor cells (EPC) transplanted into myocardial ischemia reperfusion injury region in swine.
METHODSBM-MNC marked with PKH26 (n = 9), EPC marked with CM-DiI (n = 7), phosphate buffer saline (control, n = 7) were transplanted into myocardial ischemia reperfusion injury region of swine by intracoronary artery injection. Specimens were harvested 4 weeks after injection for histological analysis (HE, immunochemical stain for vWF, alpha-sarcomeric-actin and fibronectin antibody). Cell differentiation was observed under transmission electronmicroscope.
RESULTSThe number of small blood vessels was similar between BM-MNC group and EPC group (13.39 +/- 6.96/HP vs.12.39 +/- 4.72/HP, P < 0.05), but was significantly higher than that of control group (P < 0.05). Responsive intensity of immunochemical stain for fibronectin antibody was significantly lower in BM-MNC and EPC groups than that in control group. Responsive intensity of immunochemical stain for alpha-sarcomeric-actin antibody was similar among the three groups. Cluster cells were observed in one swine from BM-MNC group which might relate to the proliferation of stem cells in situ. Immature endothelial cells and myocytes were also detected by transmission electronmicroscope in BM-MNC and EPC group.
CONCLUSIONBM-MNC and EPC transplanted into myocardial ischemia reperfusion injury region in swine stimulated the formation of blood vessels and inhibited fibrogenesis.
