Study on antiplatelet and antithrombin activitives and effective components variation of Puhuang-Wulingzhi before and after compatibility.
- Author:
Shu-lan SU
;
Ping XUE
;
Zhen OUYANG
;
Wei ZHOU
;
Jin-ao DUAN
- Publication Type:Journal Article
- MeSH:
Animals;
Antithrombins;
chemistry;
pharmacology;
Blood Platelets;
drug effects;
physiology;
Drugs, Chinese Herbal;
chemistry;
pharmacology;
Female;
Humans;
Molecular Structure;
Platelet Aggregation;
drug effects;
Rabbits;
Thrombin Time
- From:
China Journal of Chinese Materia Medica
2015;40(16):3187-3193
- CountryChina
- Language:Chinese
-
Abstract:
The changes of bioactive constituents were analyzed for Puhuang-Wulingzhi before and after compatibility and the antiplatelet and antithrombin activitives were evaluated in order to elucidate the scientific and reasonable of Puhuang-Wulingzhi compatibility. UPLC-QTOF-MA-Markerlynx, principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis were used for data analysis and tracking changes of chemical composition during the decocting process. In vitro platelet aggregation induced by ADP, thrombin time(TT) and prothrombin time (PT) were investigated for Puhuang-Wulingzhi before and after compatibility. The results showed that significant differences were found between the mixed decoction and codecoction of Wulingzhi and Puhuang. Five compounds changed obviously were identified as typhaneoside, naringenin, isorhamnetin-3-O-ruinoside, quercetin-3-O-neohesperidoside, kaempferol-3-O-neohesperidoside. The codecoction, comparing with the single decoction, was more significant in antiplatelet aggregation and could prolong thrombin time. In the same crude drug dose, the thrombin time (TT) elongation were greater. These data could provide references for elucidation of bioactive components for this herb pair.
