Isoflavonoids from Caragana changduensis and their nitric oxideinhibitory activities.
- Author:
Xiao-dong SUN
;
Shi-ming FANG
;
Mao-dan ZANG
;
Cheng-xiong YANG
;
He-ran LI
;
Susumu KITANAKA
;
Xue-dong YANG
- Publication Type:Journal Article
- MeSH:
Animals;
Caragana;
chemistry;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
pharmacology;
Isoflavones;
chemistry;
isolation & purification;
pharmacology;
Macrophages;
drug effects;
metabolism;
Mice;
Molecular Structure;
Nitric Oxide;
antagonists & inhibitors;
metabolism;
RAW 264.7 Cells
- From:
China Journal of Chinese Materia Medica
2015;40(16):3220-3223
- CountryChina
- Language:Chinese
-
Abstract:
Ten isoflavonoids were isolated from the heartwoods of Caragana changduensis Lion f. by means of various column chromatographic techniques. Based on the detailed spectral data analysis (MS and NMR), as well as comparison with the literatures, their chemical structures were determined as 7,2'-dihydroxy-8,4'-dimethoxyisoflavone (1), 4'-hydroxy-7,3'-dimethoxyisoflavone (2), 5, 7, 4'-trihydroxy-2',5'-dimethoxyisoflavone (3), prunetin (4), afrormosin (5), odoratin (6), genistein (7), texasin (8), pratensein (9), and 6,7,3'-trihydroxy-4'-methoxyisoflavone (10). Among them, compounds 1-3 and 9-10 were isolated from the Caragana genus for the first time. All the compounds were obtained from this species for the first time. In the preliminary assays, compounds 1, 2, 6, and 7 possessed significant inhibitory effects on NO production, with IC50 values of 48.12, 25.32, 62.71, 43.59 μmol x L(-1), respectively.
