Effect of evodiamine in inducing apoptosis of gastric cancer SGC-7901 cells through mTOR signal pathway.
- Author:
Xin LIU
;
Li YANG
;
Ying BI
;
Liang-hong WANG
;
Hai HUANG
- Publication Type:Journal Article
- MeSH:
Animals;
Antineoplastic Agents, Phytogenic;
pharmacology;
Apoptosis;
drug effects;
Cell Line, Tumor;
Drugs, Chinese Herbal;
pharmacology;
Humans;
Quinazolines;
pharmacology;
Signal Transduction;
drug effects;
Stomach Neoplasms;
drug therapy;
genetics;
metabolism;
physiopathology;
TOR Serine-Threonine Kinases;
genetics;
metabolism
- From:
China Journal of Chinese Materia Medica
2015;40(16):3262-3266
- CountryChina
- Language:Chinese
-
Abstract:
Evodiamine is one of the most important antitumor alkaloid from evodiamine. This study focused on the mechanism of evodiamine in inducing apoptosis of gastric cancer SGC-7901 cells through mammalian target of rapamycin (mTOR) signal pathway, in order to explore its antitumor mechanism and lay a foundation for clinical treatment of gastric cancer. The sole cytotoxic effect of evodiamine on SGC-7901 cells and human peripheral blood mononuclear cells (PBMCs) was observed by MTT assay. After the cells were respectively intervened with single evodiamine or evodiamine combined with z-VAD-fmk, the gene expressions of mTOR, p70S6K and 4EBP1 were analyzed by real-time PCR, and the protein expressions of mTOR and p-mTOR were detected by western blot. The result showed that evodiamine inhibited the apoptosis of SGC-7901 cells in a time-dependent manner, with no cytotoxic effect on human PBMCs. After the respective intervention with single evodiamine or evodiamine combined with z-VAD-fmk, the cells became round and floated in medium. Compared with the control group, both treatment methods can inhibit mTOR, 4E-BP1 and p70S6K gene expressions, with significant differences. Compared with single evodiamine, evodiamine combined with z-VAD-fmk showed a higher inhibitory rate in gene expression. According to the Western Blot result, evodiamine can inhibit the protein expressions of mTOR and p-mTOR regardless of the combination with z-VAD-fmk, with a higher inhibitory rate after z-VAD-fmk blocked caspase. In conclusion, evodiamine may promote the apoptosis of SGC-7901 cells through mTOR signal pathway.
