Study on effect of total flavanones of Sedum sarmentosum on apoptosis of hepatic stellate cells and its mechanism.
- Author:
Yuan-can LIN
;
Hai-ying LUO
;
Qian-xing JIN
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Caspase 3; genetics; metabolism; Cell Line; Cell Proliferation; drug effects; Drugs, Chinese Herbal; pharmacology; Flavanones; pharmacology; Hepatic Stellate Cells; cytology; drug effects; metabolism; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; Rats; Sedum; chemistry
- From: China Journal of Chinese Materia Medica 2015;40(16):3273-3277
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of total flavanones of Sedum sarmentosum (SSTF) on the apoptosis of rat hepatic stellate cells (HSC-T6) and its mechanism.
METHODDifferent concentrations of SSTF and HSC-T6 cells were co-cultured for different period of time. The MTT assay was used to detect the inhibitory effect of SSTF on the proliferation of HSC-T6 cells. The flow cytometry Annexin-V/PI double staining method was adopted to detect SSTF's effect on HSC-T6 cell apoptosis. Western blotting and Real-time PCR methods were applied to observe the effect on the protein and mRNA expressions of apoptosis-related cytokines Bcl-2, Bax and Caspase-3.
RESULTSSTF significantly inhibited HSC-T6 cell proliferation and induced cell apoptosis in a dose and time dependent manner. According to Western blotting result, SSTF promoted apoptosis by inhibiting Bcl-2, Bax and promoting the protein expression of Caspase-3; according to a further Real-time PCR study, Bcl-2 mRNA levels can inhibit Bcl-2 and promote Bax and Caspase-3 expressions.
CONCLUSIONSSTF has the effect of promoting the apoptosis of HSC-T6 mainly by inhibiting Bcl-2 and promoting protein and mRNA expressions of Bax and caspase-3.