Successful pregnancy following laser-assisted selection of viable but immotile spermatozoa for intracytoplasmic sperm injection: A report of 2 cases.
- Author:
Huan-hua CHEN
;
Gui-xue FENG
;
Bo ZHANG
;
Jin-hui SHU
;
Xian-you GAN
;
Hong ZHOU
;
Ruo-yun LIN
- Publication Type:Case Reports
- MeSH: Embryo Transfer; Female; Fertilization; Humans; Infertility, Male; therapy; Male; Pregnancy; Pregnancy Outcome; Sperm Injections, Intracytoplasmic; Sperm Motility; Sperm Tail; physiology
- From: National Journal of Andrology 2015;21(11):988-991
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the feasibility and clinical application value of selecting viable spermatozoa by noncontact diode laser.
METHODSWe obtained immotile spermatozoa from 2 infertile men with obstructive azoospermia or severe asthenospermia and selected viable spermatozoa using a single laser shot at the sperm tail. Those that responded to the laser shot by a curling reaction of the tail were regarded as presumably viable and used for intracytoplasmic sperm injection (ICSI).
RESULTSThe mean fertilization rate was 88.89% after ICSI with the laser-selected viable spermatozoa. Both of the embryo transfers resulted in a single pregnancy.
CONCLUSIONNoncontact diode laser is a useful alternative for the assessment of sperm viability, which may help to achieve successful pregnancy.