Expression characteristics of the Ccdc70 gene in the mouse testis during spermatogenesis.
- Author:
Jian-bo CHEN
;
Wen-zhong ZHENG
;
Yu-chi LI
;
Shou-ren LIN
;
Zeng ZHANG
;
Yong WU
;
Zhi-mao JIANG
;
Yao-ting GUI
- Publication Type:Journal Article
- MeSH: Animals; Computational Biology; Gene Expression Regulation, Developmental; Male; Mice; Proteins; genetics; Spermatogenesis; genetics; Testis; metabolism
- From: National Journal of Andrology 2016;22(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression characteristics of the gene of coiled-coil domain-containing protein 70 (Ccdc70) in the mouse testis and its potential role in spermatogenesis.
METHODSUsing expression profile microarray, we screened the mouse testis-specific gene Ccdc70, studied its expression characteristics in the mouse testis by RT-PCR, real-time PCR, Western blot and immunohistochemistry, followed by bioinformatic analysis of the Ccdc70 protein.
RESULTSThe Ccdc70 gene was expressed highly in the testis but lowly in the epididymis of the mice. The Ccdc70 protein was expressed mainly in the spermatocytes and round spermatids of the testis and in the epithelial cells of the epididymis. Bioinformatic analysis showed a structural domain in the Ccdc70 protein, which was highly conserved in mammalian evolution.
CONCLUSIONThe Ccdc70 gene is highly expressed in the mouse testis and mainly in the spermatocytes, round spermatids, and epididymal epithelial cells, which indicates that it is involved in the regulation of spermatogenesis and epididymal sperm maturation.