Enhancement effects of hypoxia on invasion and metastasis of K562 cells.
- Author:
Ying-Ming NIE
1
;
Bi-Tao DAI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Adhesion; Cell Hypoxia; Cell Movement; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; genetics; K562 Cells; Matrix Metalloproteinase 2; genetics; Matrix Metalloproteinase 9; genetics; Mice; NIH 3T3 Cells; Neoplasm Invasiveness; Neoplasm Metastasis; Vascular Endothelial Growth Factor A; genetics
- From: Chinese Journal of Contemporary Pediatrics 2009;11(7):566-570
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the potential effect of hypoxia on invasion and metastasis of leukemia cell line K562.
METHODSK562 cells were cultured with the conventional method in vitro and treated with 1%, 3% and 5% oxygen for 24 hrs. The normoxic cultured K562 cells were used as the control group. Cell adhesion assay, cell migration assay and cell invasion assay were used to detect the adhesion, migration and invasion abilities of K562 cells. RT-PCR was used to measure the mRNA expression of HIF-1alpha, VEGF, MMP-2 and MMP-9. The protein level of HIF-1alpha was measured by Western blot.
RESULTSCompared with the control group, the 3% and 5% oxygen treatment groups significantly increased the adhesion, migration and invasion abilities of K562 cells (p<0.05 or <0.01), and up-regulated the protein level of HIF-1alpha and the mRNA levels of HIF-1alpha,VEGF, MMP-9 and MMP-2 (p<0.05 or 0.01). However, there were no significant differences in the above indexes between the 1% oxygen treatment and the control groups.
CONCLUSIONSModerate hypoxia can enhance the abilities of invasion and metastasis of K562 cells, probably by an up-regulation of HIF-1alpha level and VEGF, MMP-2 and MMP-9 mRNA expression.
