Expression of human beta-defensin-3 induced by lipopolysaccharide in human bronchial epithelial cells.
- Author:
Jia LI
1
;
Bing ZHANG
;
Li-Li ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Bronchi; drug effects; metabolism; Dose-Response Relationship, Drug; Humans; Lipopolysaccharides; toxicity; RNA, Messenger; analysis; beta-Defensins; analysis; genetics
- From: Chinese Journal of Contemporary Pediatrics 2009;11(7):577-580
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of human beta-defensin-3 (hBD-3) induced by lipopolysaccharide (LPS) in human bronchial epithelial (HBE) cells, and explore the role of hBD-3 in respiratory infection.
METHODSHBE cells were stimulated with different concentrations of LPS (0.01, 0.1, 1 and 10 microg/mL). hBD-3 mRNA expression was detected by RT-PCR 2 hrs later. hBD-3 protein expression was detected by Western blot 4 hrs later.
RESULTShBD-3 mRNA and protein was weakly expressed in normal HBE cells. LPS stimulation resulted in a significant increase of hBD-3 mRNA and protein expression (p<0.01). hBD-3 mRNA and protein expression increased with increasing LPS concentrations. There were significant differences in the hBD-3 mRNA and protein expression in cells stimulated by different concentrations of LPS (p<0.05).
CONCLUSIONSLPS can induce hBD-3 expression in a dose-dependent manner. hBD-3 might play a role in initial defensive reaction against bacterial invasion.
