Effect of topotecan on MAGE gene expression of HPB-AM cells.
- Author:
Qi LEI
1
;
Suo-Qin TANG
;
Tian-You WANG
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Neoplasm; genetics; Antineoplastic Agents; pharmacology; Cell Line, Tumor; Dose-Response Relationship, Drug; Gene Expression; drug effects; Humans; Lymphoma; drug therapy; metabolism; pathology; Neoplasm Proteins; genetics; RNA, Messenger; analysis; Topotecan; pharmacology
- From: Chinese Journal of Contemporary Pediatrics 2009;11(8):679-682
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether topotecan, a novel anti-tumor agent, down-regulates gene expression of melanoma antigen-encoding (MAGE) in HPB-AM cells.
METHODSMAGE mRNA expression of HPB-AM cells was detected by RT-PCR 4, 8, 12 and 16 hrs after different concentrations (0.05, 0.10, 0.15 and 0.20 micromol/L) of topotecan treatment.
RESULTSMAGE mRNA expression of HPB-AM cells decreased with increasing concentrations of topotecan 12 hrs after treatment. The MAGE mRNA expression of HPB-AM cells treated by 0.10, 0.15 and 0.20 micromol/L of topotecan was significantly lower than that in the blank control group (P<0.05). MAGE mRNA expression of HPB-AM cells was significantly reduced in a time-dependent manner after 0.10 micromol/L of topotecan treatment. The MAGE mRNA expression of HPB-AM cells treated by 0.10 micromol/L of topotecan was significantly lower than that in the blank control group 12 and 16 hrs after treatment (P<0.05).
CONCLUSIONSTopotecan is capable of inhibiting the expression of MAGE mRNA of HPB-AM cells in a time- and dose-dependent manner.
