Relationship between glucocorticoid receptors and glucocorticoid resistance in children with idiopathic thrombocytopenia purpura.
- Author:
Ying WANG
1
;
Hui-Rong MAI
;
Lin LIN
;
Xiu-Li YUAN
;
Hong-Song SHI
;
Si-Xi LIU
;
Chang-Gang LI
Author Information
- Publication Type:Journal Article
- MeSH: Child; Child, Preschool; Drug Resistance; Female; Glucocorticoids; pharmacology; Humans; Male; Purpura, Thrombocytopenic, Idiopathic; blood; drug therapy; RNA, Messenger; analysis; Receptors, Glucocorticoid; blood; genetics
- From: Chinese Journal of Contemporary Pediatrics 2009;11(9):714-716
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the relationship between the expression of alpha and beta-isoforms of glucocorticoid receptors (GR) in peripheral blood mononuclear cells (PBMC) and glucocorticoid resistance in children with idiopathic thrombocytopenia purpura (ITP).
METHODSReal-time PCR was used to detect the expression of GR alpha and GR beta mRNA in PBMC from 30 children with ITP (glucocorticoid-sensitive, n=18; glucocorticoid-resistant, n=12) and 10 healthy children (control group). Enzyme immunoassay was used to measure plasma levels of total glucocorticoids.
RESULTSThere were no significant differences in PBMC GR alpha mRNA levels among the glucocorticoid sensitive, the glucocorticoid-resistant and the control groups. Compared with the glucocorticoid-sensitive and the control groups, the expression of GR beta mRNA in the glucocorticoid-resistant group was significantly up-regulated (p<0.01). Plasma total glucocorticoids level in the glucocorticoid-resistant group was found to be much higher than that in the glucocorticoid-sensitive and the control groups (p<0.01).
CONCLUSIONSThe up-regulated expression of GR beta mRNA may associated with glucocorticoid resistance in children with ITP.
