Enhanced immune response of a novel T-cell immunogen in vaccine for foot-and-mouth disease.
- Author:
Qing ZHAO
1
;
Pu SUN
;
Zaixin LIU
;
Pinghua LI
;
Huifang BAO
;
Yimei CAO
;
Xingwen BAI
;
Yuanfang FU
;
Zengjun LU
;
Dong LI
Author Information
1. National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
- Publication Type:Journal Article
- MeSH:
Adjuvants, Immunologic;
pharmacology;
Animals;
Capsid Proteins;
genetics;
immunology;
Epitopes, T-Lymphocyte;
genetics;
immunology;
Foot-and-Mouth Disease;
immunology;
prevention & control;
virology;
Foot-and-Mouth Disease Virus;
immunology;
Immunization;
Mice;
Viral Vaccines;
genetics;
immunology;
pharmacology
- From:
Chinese Journal of Biotechnology
2011;27(9):1281-1291
- CountryChina
- Language:Chinese
-
Abstract:
We investigated the enhanced immune response of a recombinant T cell immunogen as an effective cellular immune adjuvant. The T cell immunogen named TI contained several T cell epitopes from the VP1, VP4, 3A and 3D proteins of foot-and-mouth disease virus (FMDV) and two pan-T helper (T(H)) cell sites to broaden the immunogenicity of the protein. Meanwhile, another fusion protein named OA-VP1 was expressed in bacteria, which contained two VP1 proteins of O and Asia1 type FMDV. Mice were vaccinated with commercially inactivated vaccine or OA-VP1 protein with or without the TI immunogen. The results show that mice inoculated with inactivated vaccine or OA-VP1 protein supplemented with TI immunogen produced significantly higher level of neutralizing antibodies (P < 0.01 or P < 0.05) than the mice only inoculated with inactivated vaccine or OA-VP1 protein by microneutralization assay. An obvious increase in T cell number by flow cytometric analysis and significantly higher concentration of IFN-gamma secreted in culture media of spleen lymphocytes were observed in groups supplemented with TI immunogen (P < 0.01). TI immunogen was an effective stimulator for humoral and cellular immunity and could help improve the immunogenicity of inactivated vaccine or protein subunit vaccine.
