Effects of fructose and maltose as aerobic carbon sources on subsequently anaerobic fermentation by Escherichia coli NZN111.
- Author:
Hui WU
1
;
Zhimin LI
;
Qin YE
Author Information
1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
- Publication Type:Journal Article
- MeSH:
Aerobiosis;
Anaerobiosis;
Carbon;
metabolism;
Escherichia coli;
classification;
metabolism;
Fermentation;
Fructose;
metabolism;
Maltose;
metabolism;
Phosphotransferases;
metabolism;
Pyruvic Acid;
metabolism;
Succinic Acid;
metabolism
- From:
Chinese Journal of Biotechnology
2011;27(9):1299-1308
- CountryChina
- Language:Chinese
-
Abstract:
To understand the effects of sugar whose uptake is dependent or independent on the phosphotransferase system (PTS), two-stage culture of Escherichia coli strain NZN111 that was constructed by disruption of IdhA and pflB encoding the fermentative lactate dehydrogenase (LDH) and pyruvate: formate lyase (PFL) of E. coli W1485, was carried out for organic acids production. When NZN111 was aerobically cultured on fructose (PTS dependent) or maltose (PTS independent), it fermented glucose with succinic acid and pyruvic acid as the major products in subsequent anaerobic culture. The experiments were also performed in a 5-L fermentor. The yields of succinic acid by the fructose-and maltose-grown NZN111 were 0.84 and 0.75 mol/mol, whereas the yields of pyruvic acid were 0.65 and 0.83 mol/mol, respectively. The final ratio of succinic acid to pyruvic acid in the anaerobic stage reached 1.73:1 and 1.21:1, respectively. The different behaviors in anaerobic fermentation by the fructose-, maltose- and glucose-grown NZN111 were likely caused by the regulation of catabolite repression in the aerobic culture stage.
