Application of a pH feedback-controlled substrate feeding method in glutamic acid fermentation.
- Author:
Yu XING
1
;
Liye ZHANG
;
Wei CONG
;
Lei YUE
;
Chongan CHEN
;
Jiyin MA
Author Information
1. Collage of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
- Publication Type:Journal Article
- MeSH:
Bioreactors;
microbiology;
Corynebacterium glutamicum;
growth & development;
metabolism;
Culture Techniques;
Feedback;
Fermentation;
Glucose;
metabolism;
Glutamic Acid;
biosynthesis;
metabolism;
Hydrogen-Ion Concentration;
Quaternary Ammonium Compounds;
metabolism
- From:
Chinese Journal of Biotechnology
2011;27(10):1457-1463
- CountryChina
- Language:Chinese
-
Abstract:
A novel method based on pH value was proposed to simplify the substrate feeding method for glutamic acid fermentation. The linear relationship between the consumption amounts of ammonia (x) and that of glucose (y) was established (y = 7.4744x, R2 = 0.9989) which could be used as the ratio of the amount of ammonia and that of glucose in the feeding broth. Thus the concentration of glucose could be controlled through the adjustment of pH automatically. In the glutamic acid fermentation using the pH feedback-controlled glucose feeding method, the glucose concentration in fermentation broth was maintained between 12 and 21 g/L. Compare with the constant glucose concentration feeding method, the glucose conversion rate and glutamic acid productivity increased by 9.06% and 17.5% respectively, when the pH feedback-controlled glucose feeding method was employed, and fermentation period was shorten above 2 h.
