Effect of antibody CR6261 V(H) and scFv expression on influenza virus infection.
- Author:
Yan LI
1
;
Xiulan LI
;
Jinghua YAN
Author Information
1. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Science, Beijing 100101, China. yanjh@im.ac.cn
- Publication Type:Journal Article
- MeSH:
Antibodies, Monoclonal;
immunology;
Antibodies, Viral;
chemistry;
immunology;
Antibody Specificity;
Cell Line;
Genetic Vectors;
Hemagglutinin Glycoproteins, Influenza Virus;
chemistry;
immunology;
metabolism;
Humans;
Influenza, Human;
immunology;
Orthomyxoviridae;
drug effects;
immunology;
Single-Chain Antibodies;
immunology
- From:
Chinese Journal of Biotechnology
2011;27(10):1472-1481
- CountryChina
- Language:Chinese
-
Abstract:
The emerging CR6261 antibody could neutralize several subtype influenza virus with high affinity, whose VH domain binds to the HA protein conserved domain. Therefore, it has drawn much attention as a potential broad-spectrum therapeutic antibody against influenza virus. In this study, we constructed the eukaryotic expression vectors pCR6261V(H), pCR6261V(H)-GFP and pCR6261scFv and screened the monoclonal cell lines that could stably express CR6261V(H), CR6261V(H)-GFP and CR6261scFv on the cell membrane. After influenza virus infecting the stable cell lines, the titers of viruses were tested by hemagglutination inhibition test. The result shows that the titers of viruses in CR6261scFv and CR6261V(H)-GFP stable expression cell lines decreased and there was no obvious discrimination between the CR6261V(H) expression cell line and the negative control, suggesting that CR6261V(H) and CR6261scFv expressing on the cell membrane could partly inhibit the virus infection. Though the effective inhibition strategy is undergoing, our research will provide new clues for the breeding of anti influenza transgenic animals.
