Progress in improvement of lentiviral vectors' transcriptional read-through.
- Author:
Jiaping HE
1
;
Jingzhi ZHANG
Author Information
1. Shanghai Children's Hospital, Shanghai 200040, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Genetic Therapy;
adverse effects;
methods;
Genetic Vectors;
genetics;
Humans;
Lentivirus;
genetics;
metabolism;
Promoter Regions, Genetic;
Proto-Oncogene Proteins;
genetics;
Transcription, Genetic;
genetics;
Virus Inactivation;
Virus Integration
- From:
Chinese Journal of Biotechnology
2011;27(11):1541-1548
- CountryChina
- Language:Chinese
-
Abstract:
Four out of 10 patients of X-linked severe combined immunodeficiency (X-SCID) were finally developed leukemia after receiving the treatment of gene therapy delivered by gamma-retroviral vectors. This is due to the vector integrated to the proximity of lmo2 etc proto-oncogene promoters, leading to the activation of onco-gene expression, which raises the concern of the bio-safety of gene therapy vectors. Lentiviral vectors, especially self-inactivating lentiviral vectors, are considered to be much safer than gamma-retroviral vectors. However self-inactivating lentiviral vectors also have encountered with some unsafe factors and one of them is the problem of transcriptional "read-through" . During the past years, achievements have been made to reduce lentiviral vector transcriptional read-through, which are reviewed herein.
