Expression of a protein elicitor pebC1 from Botrytis cinerea in Pichia pastoris.
- Author:
Yunhua ZHANG
1
;
Xiufen YANG
;
Yanfeng LIU
;
Shanjiang YU
;
Dewen QIU
Author Information
1. Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
- Publication Type:Journal Article
- MeSH:
Botrytis;
chemistry;
Fungal Proteins;
biosynthesis;
genetics;
pharmacology;
Pichia;
genetics;
metabolism;
Plant Diseases;
prevention & control;
Recombinant Proteins;
biosynthesis;
genetics;
pharmacology
- From:
Chinese Journal of Biotechnology
2011;27(11):1631-1636
- CountryChina
- Language:Chinese
-
Abstract:
In order to express PebC1 in Pichia pastoris, the pebC1 sequence was amplified from genome Botrytis cinerea BC-4-2-2-1 by PCR and subcloned into the Pichua pastoris expression vector pPIC9K to generate pPIC9K-pebC1. The recombinant plasmid was linearized by Bgl II and transformed into Pichia pastoris GS115 by electroporation. Recombinant Pichia pastoris GS115/pPIC9K-pebC1 was screened by MD and G418-YPD plates and further confirmed by PCR. The protein expression was induced by methanol and analyzed by SDS-PAGE. SDS-PAGE analysis showed a special band about 39 kDa and western blotting indicated a good antigenicity of the expressed protein. Bioassay results showed that the recombinant protein PebC1 can induce resistance to gray mould disease of cucumber and Arabidopsi thaliana.
