Effects of dilution rate and carbon-to-nitrogen ratio on lipid accumulation by Rhodosporidium toruloides under chemostat conditions.
- Author:
Hongwei SHEN
1
;
Guojie JIN
;
Cuimin HU
;
Zhiwei GONG
;
Fengwu BAI
;
Zongbao K ZHAO
Author Information
1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, Liaoning, China.
- Publication Type:Journal Article
- MeSH:
Basidiomycota;
growth & development;
metabolism;
Batch Cell Culture Techniques;
Carbon;
metabolism;
Culture Media;
Fatty Acids;
metabolism;
Glucose;
metabolism;
Lipids;
biosynthesis;
Nitrogen;
metabolism;
Oleic Acid;
biosynthesis;
Palmitic Acid;
metabolism
- From:
Chinese Journal of Biotechnology
2012;28(1):56-64
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this work is to investigate how dilution rate and carbon-to-nitrogen (C/N) ratio affects lipid accumulation by Rhodosporidium toruloides AS 2.138 9 in continuous culture. Under steady-state conditions, the increase in dilution rate led to the decrease in lipid content and lipid yield. The highest lipid yield and lipid content at D = 0.02 h(-1) were 0.18 g lipid/g sugar and 57.1%, respectively, while the highest lipid productivity and biomass productivity were obtained at D = 0.14 h(-1). The increase in C/N ratio led to the increase in lipid content. The highest lipid content of 38% was obtained at C/N = 237. The highest lipid yield of 0.12 g lipid/g sugar was obtained at C/N = 92. However, the highest lipid productivity of 0.12 g/(L x h) was obtained at C/N = 32. No significant changes were observed in terms of fatty acid composition of the lipid produced under different C/N ratios, and these three fatty acids, palmitic acid, stearic acid and oleic acid, took over 85% in all samples.
