Analysis of an acellular pigskin based nerve scaffold.
- Author:
Bin LIU
1
;
Jinxing KE
;
Shaoxi CAI
;
Xiaokun LI
;
Lu ZHANG
;
Wenqi CHEN
;
Yaoguang ZHANG
Author Information
1. Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Chongqing 400715, China.
- Publication Type:Journal Article
- MeSH:
Acellular Dermis;
Animals;
Biocompatible Materials;
Guided Tissue Regeneration;
Lactic Acid;
pharmacology;
Lysine;
pharmacology;
Nerve Growth Factors;
chemistry;
pharmacology;
Nerve Regeneration;
Polyglycolic Acid;
pharmacology;
Swine;
Tissue Engineering;
Tissue Scaffolds
- From:
Chinese Journal of Biotechnology
2012;28(3):349-357
- CountryChina
- Language:Chinese
-
Abstract:
A scaffold fabricated with lysine/nerve growth factor (NGF)/poly (lactic acid coglycolic acid) copolymer (PLGA) and acellular pigskin was evaluated in vitro as a potential artificial nerve scaffold. Properties of the scaffold such as microstructure, mechanical property, degradation behavior in PBS and water, Schwann cell adhesion property, and release of NGF were investigated. Results showed PLGA had permeated into the porous structure of acellular pigskin; its breaking strength was 8.308 MPa, breaking extensibility was 38.98%, elastic modulus was 97.27 MPa. The porosities of the scaffold ranged from 68.3% to 81.2% with densities from 0.62 g/cm3 to 0.68 g/cm3. At 4 weeks of degradation in vitro, maximum mass loss ratio was 43.3%. The release of NGF could still be detected on the 30th day, and its accumulative release rate was 38%. Lysine added into the scaffold neutralized the acidoid preventing degradation of PLGA to maintain a solution pH value. Schwann cells had grown across the scaffold after co-cultivation for 15 days. These in vitro properties of the pigskin based composite might indicate its potentiality to be an artificial nerve scaffold.
