Establishment of Mongolian gerbil model of gastric cancer induced by Helicobacter pylori infection and its proteomics analysis.
- Author:
Yan ZHAO
1
;
Yuan XIE
;
Xian CHEN
;
Wenjie XU
;
Yan WANG
;
Jianjiang ZHOU
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Gastritis; microbiology; pathology; Gerbillinae; Helicobacter Infections; complications; metabolism; Helicobacter pylori; genetics; Humans; L-Lactate Dehydrogenase; metabolism; Male; Metaplasia; Microfilament Proteins; metabolism; Muscle Proteins; metabolism; Proteomics; Proton-Translocating ATPases; metabolism; RNA, Ribosomal, 16S; analysis; Stomach Neoplasms; metabolism; microbiology; Tandem Mass Spectrometry
- From: Chinese Journal of Pathology 2014;43(12):820-826
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an animal model of gastric cancer by long-term infection of Helicobacter pylori (H.pylori) and to elucidate the pathogenesis by proteomics analysis.
METHODSFifty male Mongolian gerbils (4-5 week-old and weighted 60-100 g) were infected with H.pylori and the gastric tissues were obtained after the infection at 3, 6, 12 and 24 months. Histological changes were evaluated by H-E staining of the gastric tissue sections. Detection of H.pylori was performed by in-vitro culture of fresh gastric tissue samples, PCR amplification of H.pylori 16s rRNA and localization by silver staining. In addition, proteins extracted from gastric tissue samples were subjected to two-dimensional electrophoresis (2-DE) at various infection time points. Protein spots with increased quantity over the course of H.pylori infection were selected and analyzed by LC-MS/MS. Finally, differentially expressed proteins between human gastric cancer tissue samples and lymph nodes were analyzed by real-time RT-PCR.
RESULTSColonization of H.pylori was observed in gastric tissue of gerbils as early as 3 months after H.pylori infection, and persisted till 24 months. Pathological examination of infected animals showed various histological changes including acute gastritis, atrophic gastritis, intestinal metaplasia and gastric carcinoma. Seventy-eight differentially expressed proteins were identified by proteomics analysis, among which 36 proteins were up-regulated and 42 were down-regulated. Analyzed by LC-MS/MS, ten proteins were identified, including lactate dehydrogenase, ATP synthase, fatty acid-binding protein, COX5B, peroxiredoxin-4, peroxide reductase, transgelin, succinyl-CoA ligase, keratin and protein disulfide-isomerase A2, among which transgelin, ATP synthase and lactate dehydrogenase were highly expressed in human gastric carcinoma and lymph nodes.
CONCLUSIONSH.pylori infection induces the expression of transgelin, ATP synthase and lactate dehydrogenase, implying possible roles in the pathogenesis of gastric diseases including cancer.
