Cannabinoid receptor 1 controls nerve growth in ectopic cyst in a rat endometriosis model.
- Author:
Qianqian ZHAO
1
;
Xizi LIANG
1
;
Hongxiu HAN
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Cysts; metabolism; Disease Models, Animal; Endometriosis; metabolism; Female; Peripheral Nerves; growth & development; metabolism; Piperidines; pharmacology; Pyrazoles; pharmacology; Rats; Receptor, Cannabinoid, CB1; antagonists & inhibitors; physiology; Ubiquitin Thiolesterase; metabolism
- From: Chinese Journal of Pathology 2014;43(12):827-830
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether cannabinoid receptor 1 (CB1R) is involved in nerve growth in endometriosis-associated ectopic cyst.
METHODSThe effect of CB1R agonist and antagonist on the expression of pan-neuronal marker protein gene product (PGP) 9.5 in ectopic cyst was examined by immunofluorescence and Western blot in endometriosis model of 18 rats.
RESULTSImmunofluorescence revealed that PGP 9.5 was expressed in the nerve fibers and was mainly distributed in the cyst hilum. Western blot revealed that the protein density of either PGP 9.5 (2 week: 0.38 ± 0.05; 4 week: 0.63 ± 0.03; 8 week: 0.80 ± 0.07, P < 0.01) or CB1R (2 week: 0.48 ± 0.04; 4 week: 0.68 ± 0.01; 8 week: 0.80 ± 0.03, P < 0.01) in the ectopic cyst increased with cyst size. In addition, compared to control group (0.75 ± 0.01), PGP 9.5 expression in the ectopic cyst was promoted by CB1R agonist ACPA (0.81 ± 0.01, P < 0.05), and inhibited by CB1R antagonist AM251 (0.67 ± 0.03, P < 0.01).
CONCLUSIONSCB1R was involved in the nerve growth of ectopic cyst associated with endometriosis.
