Effect of Panax notoginseng saponins on human kidney fibroblast.
- Author:
Ying WEI
1
;
Jun-ming FAN
;
Li-ping PAN
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Collagen Type I; metabolism; Fibroblasts; drug effects; Fibrosis; prevention & control; Humans; Integrin beta1; metabolism; Kidney; cytology; pathology; Panax; chemistry; Saponins; pharmacology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2002;22(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Panax notoginseng saponins (PNS) on cell proliferation, type I collagen secretion and integrin beta 1 expression in human kidney fibroblast (KFB).
METHODSKFB were cultured and stimulated by PNS in vitro. The cell proliferation, type I collagen secretion and integrin beta 1 expression in KFB were measured by methyl thiazolyl tetrazolium (MTT), enzyme-linked immunoabsorbent assay (ELISA) and flowcytometry respectively.
RESULTSWithin its optimal concentration range and effective time range, PNS could obviously inhibit the proliferation, type I collagen secretion and integrin beta 1 expression (all P < 0.05) in human KFB.
CONCLUSIONPNS would possibly be an effective drug for renal interstitial fibrosis prevention and treatment.
