Effect of bushen yizhi recipe on neurotransmitter release in A beta segment neurotoxin induced NG108-15 cellular model of Alzheimer disease.
- Author:
Zhen-guo ZHONG
1
;
Mao-cai LIU
;
Shi-long LAI
Author Information
- Publication Type:Journal Article
- MeSH: Alzheimer Disease; metabolism; pathology; Amyloid beta-Peptides; metabolism; Animals; Cell Line; Choline O-Acetyltransferase; metabolism; Drugs, Chinese Herbal; pharmacology; Neurotoxins; pharmacology; Neurotransmitter Agents; metabolism; Random Allocation; Rats; Rats, Wistar; Synapsins; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2002;22(1):50-53
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Bushen Yizhi Recipe (BSYZR) on neurotransmitter release in A beta segment neurotoxin induced NG108-15 cellular model of Alzheimer disease (AD).
METHODSThe levels of choline acetyltransferase (ChAT) activity, synapsin and functional synapse formation rate in the cellular model treated with BSYZR containing serum were determined by Western blot analysis, immunoradiometric assay and electrophysiologic technique.
RESULTSBSYZR containing serum treatment could cause increase of ChAT activity and synapsin level in model cells, as compared with those in normal control model cells treated with non-drug containing serum, it also could regulate the release capacity of transmitter and raise the functional synapse formation.
CONCLUSIONBSYZR could reduce the reaction of cell to A beta neurotoxin, indicating that it could be antagonistic to the pathological development of AD by means of raising the neurotransmitter release capacity.
