Effect of congsheng capsule on intracellular calcium concentration in mice after ischemic cerebral injury.
- Author:
Ling ZHAO
1
;
Qiu-ping XU
;
Lin LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; metabolism; Brain Ischemia; metabolism; Calcium; metabolism; Calcium Channel Blockers; therapeutic use; Capsules; Drugs, Chinese Herbal; therapeutic use; Female; Intracellular Fluid; metabolism; Male; Mice; Neuroprotective Agents; therapeutic use; Phytotherapy; Rats; Rats, Wistar; Reperfusion Injury; metabolism; Synaptosomes; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2003;23(4):281-283
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Congsheng Capsule (CSC) in intracellular Ca2+ concentration in cerebral intra-synaptosomes of ischemia/reperfusion mice and analogous ischemic injured neurons to estimate the mechanism of CSC in antagonizing ischemic cerebral injury.
METHODSThe concentration of Ca2+ in cerebral intra-synaptosomes of mice and in neurocyte suspension of fetal mice were determined using Fura-2/AM double wavelength fluorescence spectrophotometer.
RESULTSCSC 3 g/kg and 9 g/kg through gastrogavage could relieve the degree of calcium overloading of intra-synaptosomes in mice after cerebral ischemia/reperfusion. The CSC containing serum could inhibit the elevation of Ca2+ content in neurocytes induced by 1 hour hypoxia and glutamate of high concentration (200 mumol/L).
CONCLUSIONCSC plays a role in antagonizing ischemic injury through relieving the calcium overloading after cerebral ischemia.
