Detection of binding of three aldehyde compounds with DNA using high performance liquid chromatograph.
- Author:
Bin FENG
1
;
Hua SHAO
;
Hao ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Acetaldehyde; chemistry; toxicity; Acrolein; chemistry; toxicity; Aldehydes; chemistry; toxicity; Chromatography, High Pressure Liquid; DNA; chemistry; DNA Damage; drug effects; Formaldehyde; chemistry; toxicity; Guanosine Monophosphate; chemistry
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(2):86-88
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the binding sites and characteristics of the adduct from the reaction of formaldehyde, acetaldehyde, acrolein with DNA.
METHODSFormaldehyde, acetaldehyde, acrolein were reacted with four kinds of deoxyribonucleoside monophosphate (dNMP) in buffered solutions with neutral pH. The reaction products were separated by high performance liquid chromatograph (HPLC) and characterized by UV spectroscopy.
RESULTSThe reaction of formaldehyde, acetaldehyde with dG was separated and detected by HPLC. The reaction of acrolein, formate, acetic acid, Mercapturic acid with dG was not separated and detected by HPLC, while the dominant dNMP binding with formaldehyde, acetaldehyde was also determined.
CONCLUSIONFormaldehyde, acetaldehyde could bind with dGMP to express genotoxic effects.
