DMF-induced adult hepatocyte apoptosis and its effects on expression of Bcl-2, Bax and Caspase-3.
- Author:
Yan-Yan LU
;
Zhi-Qiang XUAN
;
Xue-Zhi LI
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Caspase 3; metabolism; Cells, Cultured; Dimethylformamide; pharmacology; Hepatocytes; drug effects; metabolism; pathology; Humans; Proto-Oncogene Proteins c-bcl-2; metabolism; bcl-2-Associated X Protein; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(5):276-279
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the apoptosis-inducing effect of DMF on the human liver cells (HL-7702) in vitro.
METHODSLiver cells were exposed to different concentrations of DMF (0, 50, 100, 150, 200 mmol/L) for 12 hours. Apoptotic rate, the expression of Bax, Bcl-2 and Caspase-3 in liver cells were measured by FCM and western blotting respectively.
RESULTSThe increase in apoptotic rate of hepatocytes in concentration-manner was shown after DMF treatment for 12 h. After treatment the expression of Bcl-2 was decreased steadily and lower than the control group (P < 0.01), the expression of Bax showed no significant difference among the groups of different dosage by one-factor analysis of variance (P > 0.05), as the increase of the dosage of DMF. The ratio of Bcl-2/Bax dropped with the dosage of DMF increasing, and the ratio in 200 mmol/L of DMF was significantly lower than that of the control (P < 0.01). The new lands of procaspase-3 in 150, 200 mmol/L were observed, which demonstrated that there was active caspase-3.
CONCLUSIONDMF can induce apoptosis of cultured adult normal hepatocytes in vitro, and the mechanism might be related to the decrease of Bcl-2/Bax and the cleavage of Caspase-3.