Effects of astragalus and its active ingredients on ischemia reperfusion injury in isolated guinea-pig heart
10.3760/cma.j.issn.0253-3758.2014.09.011
- VernacularTitle:黄芪及其活性成分对离体豚鼠心脏的作用
- Author:
Haining ZHANG
1
;
Dongyu MIN
;
Mingyu FU
;
Jing TIAN
;
Qingwen WANG
;
Xinjiang AN
Author Information
1. 221000,徐州市儿童医院心血管内科
- Keywords:
Myocardial reperfusion injury;
Astragalus membranaceus;
Pharmacologic actions
- From:
Chinese Journal of Cardiology
2014;42(9):759-764
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of astragalus (AST),total flavone of astragalus (TFA),total saponins of astragalus (TSA) and astragalus polysaccharides (APS) on ischemia/reperfusion (40 min/60 min) injury in isolated guinea-pig heart.Methods Isolated guinea-pig hearts underwent ischemia,then followed by K-H perfusion (I/R group),AST (60 mg/L),AST (60 mg/L),TFA (60mg/L),TSA (60 mg/L) and APS (60 mg/L) perfusion (n =6 each).Isolated hearts without ischemia serve as control group (n =6).Activity of lactate dehydrogenas (LDH) and creatine kinase (CK) in effluent were measured.Infarct size,myocardial superoxide dismutase (SOD) activity and malondiadehyde (MDA) contents were also determined.Results Compared to control hearts,heart rate,coronary flow and myocardial superoxide dismutase (SOD) activity were significantly reduced,while LDH and CK in effluent as well as myocardial MDA were significantly increased in the I/R hearts during reperfusion (all P ＜ 0.05),these changes could be partly reversed by AST and TFA perfusion.Infarct size was also significantly reduced in AST (11.9 ± 2.03) ％ and TFA (13.31 ± 1.17) ％ treated hearts compared to that in I/R group(18.9 ±2.27) ％ (all P ＜ 0.01).Conclusions The findings indicate that AST and TFA could attenuate I/R injury in isolated guinea-pig heart possibly through enhancing the activity of SOD and reducing lipid peroxidation.
