Slfn1 inhibited the migration of endothelial progenitor cells in vitro
10.3760/cma.j.issn.0253-3758.2014.11.015
- VernacularTitle:睡眠因子1负性调控内皮祖细胞迁移的体外研究
- Author:
Lu ZHANG
1
;
Chunyan KUANG
;
Tianhe YANG
;
Qiang WU
;
Yang ZHANG
Author Information
1. 贵州省人民医院体检中心
- Keywords:
Endothelium,vascular;
Stem cells;
Cell movement;
Slfn1
- From:
Chinese Journal of Cardiology
2014;42(11):951-956
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Schlafen 1 (Slfn1) on the migration of endothelial progenitor cells (EPCs).Methods Rat bone marrow derived EPCs were isolated and cultured.Ad-Slfn1,ShRNA-Slfn1,ShRNA-control and Ad-control were transfected into EPCs respectively.The mRNA expression of Slfn1 and Cyclin D1 was examined by reverse transcriptase-PCR,and their protein expression was detected by Western blot.The migration of EPCs was examined by a modified Boyden chamber assay.EPCs cell cycle was determined using flow cytometry analysis.Results Forty-eight hours after ShRNA-Slfn1 transfection,the mRNA and protein expression of Slfn1 in EPCs was significantly down-regulated compared to ShRNA-control EPCs (P ＜ 0.05).Transfection of Ad-Slfn1 reversed these changes.Overexpression of Slfn1 reduced the migration capacity of EPCs while the silencing of Slfn1 by shRNA-Slfn1 increased the migration capacity of EPCs.In addition,cell cycle was arrested at G1 phase in Slfn1 overexpression group while transfection of shRNA-Slfn1 reversed these responses.Interestingly,the mRNA and protein expression of Cyclin D1 was significantly up-regulated after shRNA-Slfn1 transfection compared to ShRNA-control group (all P ＜ 0.05),but overexpression of Slfn1 reversed these results,suggesting Cyclin D1 was involved in regulating EPCs cell cycle via Slfn1 signaling.Conclusions Slfn1 could reduce the migration capacity of EPCs via Cyclin D1 pathway.
