An experimental study on the relationship between the extracellular matrix and apoptosis of intestinal epithelium after burn injury.
- Author:
Yun ZHAO
1
;
Feng-jun WANG
;
Pei WANG
;
Shi-liang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Burns; metabolism; pathology; Caspase 3; metabolism; Collagen Type IV; metabolism; Extracellular Matrix; metabolism; Female; Intestinal Mucosa; metabolism; pathology; Laminin; metabolism; Male; Rats; Rats, Wistar
- From: Chinese Journal of Burns 2004;20(4):214-216
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between the extracellular matrix and apoptosis of intestinal epithelium after burn injury.
METHODSThirty Wistar rats were employed in the study and were randomly divided into normal control (C) and 6 PBH (postburn hour), 12 PBH, 1 PBD (postburn day), 3 PBD and 5 PBD group with 5 rats in each group. The rats in burn groups were sacrificed at 0, 6 and 12 PBHs and 1, 3 and 5 PBDs. The apoptotic cell count and the caspases-3 activity of intestinal mucosal epithelium, and the extracellular matrix component laminin and type IV collagen were determined and their correlation was analyzed.
RESULTSThe apoptotic cell count and the caspases-3 activity of intestinal mucosal epithelium in burn groups were obviously higher than those in C group (P < 0.05 or 0.01), while the intestinal mucosal contents of laminin and type IV collagen in burned groups were much lower than those in C group (P < 0.05 or 0.01). By linear correlative analysis, it was shown that the changes in the intestinal mucosal contents of laminin and type IV collagen in burned groups were negatively correlated with the change in apoptotic cell count (r = -0.575, -0.613, P < 0.05).
CONCLUSIONIntestinal epithelial apoptosis was enhanced after burn injury, and it was correlated with the change in the components of the extracellular matrix.
