Study on the anti-maturation features of immature dendritic cells induced by low dose of granulocyte macrophage colony stimulating factor.
- Author:
Qiang WANG
1
;
Yi-zhi PENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; drug effects; immunology; Cell Differentiation; drug effects; Cells, Cultured; Dendritic Cells; drug effects; immunology; Granulocyte-Macrophage Colony-Stimulating Factor; pharmacology; Interferon-alpha; pharmacology; Lipopolysaccharides; pharmacology; Lymphocyte Activation; Lymphocyte Culture Test, Mixed; Mice; Mice, Inbred BALB C; Spleen; cytology; immunology; T-Lymphocytes; cytology; immunology; Tumor Necrosis Factor-alpha; pharmacology
- From: Chinese Journal of Burns 2004;20(6):327-329
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of maturative agents, including lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF-alpha) and interferon gamma(IFN-gamma) on the maturation of immature dendritic cells originated from murine bone marrow induced by low dose of granulocyte macrophage colony stimulating factor (rm GM-CSF).
METHODSDendritic cells from murine bone marrow progenitors were cultured in low and high doses of GM-CSF for 6 days, and then the suspending cells were harvested for the experiment. After 3 days of co-culture of the obtained DC with low dose rmGM-CSF (GM(low)DC) with LPS, TNF-alpha and IFN-gamma, the stimulatory capacity of inducing proliferation of non-sensitized splenocytes of GM(low)DC in mixed lymphocyte reaction (MLR) was observed and compared with that of GM(high)DC.
RESULTSGM(low)DC could not activate the non-sensitized splenocytes or induce it into proliferation after 3 days of co-incubation with LPS, TNF-alpha, IFN-gamma, with the stimulation index (SI) lower than 2. Whereas GM(high)DC could strongly activate naive splenocytes (SI = 4.71).
CONCLUSIONGM(low)DC was resistant to maturation and insensitive to the stimulation by LPS, TNF-alpha or IFN-gamma.
