Changes in the expression of apoptotic genes in the intestinal tissue of scalded rats before and after resuscitation.
- Author:
Jing CHEN
1
;
Jia-Han WANG
;
Jia-Liang REN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Burns; metabolism; pathology; Gene Expression; Genes, fos; Intestinal Mucosa; metabolism; Intestine, Small; metabolism; pathology; Male; Proliferating Cell Nuclear Antigen; metabolism; Rats; Rats, Wistar; Reperfusion Injury; metabolism; pathology; Shock, Traumatic; bcl-2-Associated X Protein; metabolism
- From: Chinese Journal of Burns 2005;21(1):55-56
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the dynamic changes in the expression of c-fos, proliferation cell nuclear antigen (PCNA) and Bax in the intestinal tissue of scalded rats before and after resuscitation.
METHODSWistar rats inflicted with 30% TBSA full thickness scald were employed as the model and randomly divided into four groups with 8 in each group, i.e. 2.0, 2.5, 4.0, 6.0 postscald hour (PSH) groups. Rats in each group received routine fluid infusion at 2 PSH, and were sacrificed at 2, 2.5, 4, 6 PSH, respectively. Then the intestinal tissue of the rats was harvested for the detection of the expression of c-fos, PCNA and Bax.
RESULTSThe expression of c-fos, PCNA and Bax at 2.0 PSH group (65.8 +/- 4.2%, 74.5 +/- 2.4%, 26.3 +/- 5.7%, respectively) significantly increased when compared with those in 2.5 PSH group (92.4 +/- 5.7%, 85.6 +/- 4.5%, 67.1 +/- 6.6%, respectively) (P < 0.01). The expression of 3 genes increased dramatically at 2.5 and 4.0 PSH, and reached the peak at 2.5 PSH. There was no obvious difference in the gene expression between 4 PSH and 2 PSH groups.
CONCLUSIONThe expression of apoptotic genes in the intestinal tissue of scalded rats increased significantly during early resuscitation stage after burn injury.
