The role of myeloid differentiation protein-2 in lipopolysaccharide-induced cellular activation of human endothelial cells.
- Author:
Jian-qiong XIONG
1
;
Pei-fang ZHU
;
Zheng-guo WANG
;
Jian-xin JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; Cells, Cultured; Endothelial Cells; metabolism; Humans; Interleukin-6; genetics; Interleukin-8; metabolism; Lipopolysaccharides; pharmacology; NF-kappa B; metabolism; Umbilical Veins; cytology
- From: Chinese Journal of Burns 2005;21(2):97-99
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of myeloid differentiation protein-2 (MD-2) in the human endothelial cells and its role in lipopolysaccharide (LPS)-induced NF-kappaB activation in endothelial cells.
METHODSIn vitro cultured human umbilical vein endothelial cells (HUVEC) were employed in the study. The expression of MD-2 mRNA and protein, and the effect of LPS on the expression of its mRNA and protein were assessed with RT-PCR and Western blotting. The role of MD-2 in LPS-induced NF-kappaB activation and IL-8 production were investigated with gene transfection of mutant MD-2 cDNA (0.5, 1.0, 2.0 microg), pEF-BOS vacant vector (2.0 microg) and MD-2 plasmid (2.0 microg) into HUVEC, respectively.
RESULTSThere was MD-2 mRNA and protein expression in HUVECs before LPS stimulation, and it could be obviously upregulated by LPS in time and dose-dependent manner (MD-2 protein absorbency was 25 196 +/- 1 723 without LPS stimulation, which was obviously lower than that stimulated with 0.01 mg/L LPS (58 817 +/- 3 241, P < 0.01) for 6 hours. Transfection of mutant MD-2 cDNA could remarkably inhibit LPS-induced NF-kappaB activation and IL-8 production in endothelial cells.
CONCLUSIONMD-2 might play an important role in the LPS-induced NF-kappaB activation in HUVECs.
