Influence of dermal template on the biomechanical compliance of wound tissue.
- Author:
Ying-kai LIU
1
;
Shu-liang LU
;
Chun QING
;
Zhi-long CHEN
;
Jun XIANG
;
Ting XIE
;
Liang QIAO
;
Wei-dong LIN
;
Qing-shen HU
;
Zhen-jiang LIAO
;
Ji-xiang SHI
Author Information
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Animals; Compliance; Dermis; metabolism; Disease Models, Animal; Male; Rats; Rats, Sprague-Dawley; Skin Transplantation; Skin, Artificial; Soft Tissue Injuries; physiopathology; surgery; Wound Healing
- From: Chinese Journal of Burns 2005;21(2):122-124
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of dermal template on the biomechanical compliance of wound tissue during wound repair.
METHODSOne hundred and forty-four SD rats subjected to full-thickness skin loss on the dorsum were enrolled in the study, and they were randomly divided into A (n = 6, without grafting on wound), B (n = 6, with full thickness skin grafting on wound), C (n = 6, with razor thin skin grafting on wound) and D [n = 6, with acellular dermal matrix (ADM) and razor thin skin grafting on wound] groups. The tissue samples from the wounds were harvested 1, 2, 4, 6, 12 and 20 weeks after the operation. The biomechanical compliance of the wound was assessed by Instron biomechanics tensiometer. The expression of alpha-SMA in the dermal fibroblasts of each group was determined by immunohistochemistry (ABC) method.
RESULTSThe biomechanical compliance of the wound in D group was higher than that in A and C groups (P < 0.05), but lower than that in B group during 4 to 20 weeks after operation (P < 0.05). The expression of alpha-SMA in D group (7.53 +/- 0.98)% was lower than that in A (26.99 +/- 2.90)% and C (2.18 +/- 2.79)% groups (P < 0.01), but higher than that in B group at 4 weeks after operation (P < 0.05).
CONCLUSIONDermal template might affect the scar formation during wound healing, in improving wound healing quality by enhancing the biomechanical compliance of wound tissue.
