Chromatographic finger print analysis of steroids in Aerva lanata L by HPTLC technique.
- Author:
Mariswamy YAMUNADEVI
1
;
Edward Gnaraj WESELY
;
M JOHNSON
Author Information
- Publication Type:Journal Article
- Keywords: Aerva lanata L.; Fingerprint; HPTLC profile; Steroids
- MeSH: Amaranthaceae; chemistry; Chromatography, Thin Layer; methods; Humans; Plant Extracts; chemistry; isolation & purification; Plants, Medicinal; chemistry; Steroids; analysis
- From:Asian Pacific Journal of Tropical Biomedicine 2011;1(6):428-433
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo determine the chemical profile and steroids composition of the medicinally important plant Aerva lanata (A. lanata) L.
METHODSPreliminary phytochemical screening was done by the method as Harborne described. HPTLC studies were carried out as Harborne and Wagner et al described. The Ethyl acetate-ethanol-water (8: 2: 1.2) was employed as mobile phase for glycosides.
RESULTSThe desired aim was achieved using Chloroform-acetone (8: 2) as the mobile phase. The methanolic extract of stem, leaves, root, flower and seeds of A. lanata showed the presence of 30 different types of steroids with 30 different Rf values from 0.04 to 0.97. Maximum number (11) of steroids has been observed in leaves followed by root (10).
CONCLUSIONSHPTLC profile of steroids has been chosen here to reveal the diversity existing in A. lanata. Such finger printing is useful in differentiating the species from the adulterant and act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies.
