In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe(2+)-induced lipid peroxidation in rat pancreas.
- Author:
Stephen Adeniyi ADEFEGHA
1
;
Ganiyu OBOH
Author Information
- Publication Type:Journal Article
- Keywords: Alpha-amylase; Alpha-glucosidase; Antioxidant; Fe2+-induced lipid peroxidation Pancrea; Phenol
- MeSH: Animals; Antioxidants; chemistry; Carbohydrate Metabolism; drug effects; Diabetes Mellitus, Type 2; metabolism; Dose-Response Relationship, Drug; Enzyme Inhibitors; chemistry; pharmacology; Ferrous Compounds; pharmacology; Flavonoids; chemistry; Glycoside Hydrolase Inhibitors; chemistry; pharmacology; Inhibitory Concentration 50; Lipid Peroxidation; drug effects; Pancreas; drug effects; metabolism; Phenols; chemistry; Plant Extracts; chemistry; pharmacology; Polyphenols; chemistry; pharmacology; Rats; Syzygium; chemistry; alpha-Amylases; antagonists & inhibitors; alpha-Glucosidases
- From:Asian Pacific Journal of Tropical Biomedicine 2012;2(10):774-781
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate and compare the inhibitory properties of free and bound phenolic extracts of clove bud against carbohydrate hydrolyzing enzymes (alpha-amylase & alpha-glucosidase) and Fe(2+)-induced lipid peroxidation in rat pancreas in vitro.
METHODSThe free phenolics were extracted with 80% (v/v) acetone, while bound phenolics were extracted from the alkaline and acid hydrolyzed residue with ethyl acetate. Then, the interaction of the extracts with alpha-amylase and alpha-glucosidase was subsequently assessed. Thereafter, the total phenolic contents and antioxidant activities of the extracts were determined.
RESULTSThe result revealed that both extracts inhibited alpha-amylase and alpha-glucosidase in a dose-dependent manner. However, the alpha-glucosidase inhibitory activity of the extracts were significantly (P<0.05) higher than their alpha-amylase inhibitory activity. The free phenolics (31.67 mg/g) and flavonoid (17.28 mg/g) contents were significantly (P<0.05) higher than bound phenolic (23.52 mg/g) and flavonoid (13.70 mg/g) contents. Both extracts also exhibited high antioxidant activities as typified by their high reducing power, 1,1 diphenyl-2- picrylhydrazyl (DPPH) and 2, 2-azinobis-3-ethylbenzo-thiazoline-6-sulfonate (ABTS) radical scavenging abilities, as well as inhibition of Fe(2+)-induced lipid peroxidation in rat pancreas in vitro.
CONCLUSIONSThis study provides a biochemical rationale by which clove elicits therapeutic effect on type 2 diabetes.
