Evaluation of in-vitro antibacterial activity and anti-inflammatory activity for different extracts of Rauvolfia tetraphylla L. root bark.
- Author:
B Ganga RAO
1
;
P Umamaheswara RAO
;
E Sambasiva RAO
;
T Mallikarjuna RAO
;
V S Praneeth D
Author Information
- Publication Type:Journal Article
- Keywords: Anti-inflammatory activity; In-vitro antibacterial activity; Inflammation; Rauvolfia tetraphylla; Root bark
- MeSH: Animals; Anti-Bacterial Agents; pharmacology; Anti-Inflammatory Agents; pharmacology; Bacteria; drug effects; Carrageenan; adverse effects; Disease Models, Animal; Edema; chemically induced; drug therapy; Female; Male; Microbial Sensitivity Tests; Plant Bark; chemistry; Plant Extracts; pharmacology; Plant Roots; chemistry; Rats; Rauwolfia; chemistry
- From:Asian Pacific Journal of Tropical Biomedicine 2012;2(10):818-821
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo assess the in-vitro antibacterial activity and anti-inflammatory activity of orally administered different extracts (Hydro-alcoholic, methanolic, ethyl acetate and hexane) of Rauvolfia tetraphylla (R. tetraphylla) root bark in Carrageenan induced acute inflammation in rats.
METHODSIn-vitro antibacterial activity was evaluated for extracts against four Gram positive and four Gram negative bacteria by using cylinder plate assay. Hydro-alcoholic extract (70% v/v ethanol) at 200, 400 and 800 mg/kg doses and methanolic, ethyl acetate and hexane extracts at doses 100, 200 and 400 mg/kg were tested for anti-inflammatory activity in Carrageenan induced rat paw oedema model and paw thickness was measured every one hour up to 6 hrs.
RESULTSAll extracts of R. tetraphylla root bark showed good zone of inhibition against tested bacterial strains. In Carrageenan induced inflammation model, hydro-alcoholic and methanolic extract of R. tetraphylla root bark at three different doses produced significant (P<0.001) reduction when compared to vehicle treated control group and hexane, ethyl acetate extracts.
CONCLUSIONSIn the present study extracts of R. tetraphylla root bark shows good in-vitro antibacterial activity and in-vivo anti-inflammatory activity in rats.
