Comparative study between primary mediastinal B-cell lymphoma and non-mediastinal diffuse large B-cell lymphoma by immunoglobulin gene rearrangement and Epstein-Barr virus infection detection.
- VernacularTitle:原发性纵隔B细胞淋巴瘤与非纵隔弥漫性大B细胞淋巴瘤在基因重排检测与EB病毒感染方面的对比研究
- Author:
Ding-rong ZHONG
1
;
Qing LING
;
Xiao-hua SHI
;
Zhi-yong LIANG
;
Tong-hua LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Epstein-Barr Virus Infections; Female; Gene Rearrangement, B-Lymphocyte; Herpesvirus 4, Human; genetics; isolation & purification; Humans; In Situ Hybridization; Lymphoma, B-Cell; genetics; virology; Lymphoma, Large B-Cell, Diffuse; classification; genetics; virology; Male; Mediastinal Neoplasms; genetics; virology; Middle Aged; RNA, Viral; analysis; Retrospective Studies; Young Adult
- From: Chinese Journal of Pathology 2012;41(6):361-365
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differences between primary mediastinal B-cell lymphoma (PMBCL) and non-mediastinal conventional diffuse large B-cell common lymphoma (DLBCL) in immunoglobulin gene rearrangement and EB virus infections.
METHODSTwenty cases of PMBCL and 30 cases of non-mediastinal DLBCL were collected from September, 2000 to May, 2011. Pathological data were retrospectively analysed. Immunoglobulin heavy chain and light chain gene rearrangements and EBER in-situ hybridization were performed.
RESULTSSix of 20 cases of PMBCL showed monoclonal gene rearrangement, all of which were weakly detected. Twenty-seven of 30 cases of ordinary diffuse large B-cell lymphoma showed monoclonal gene rearrangement, which were strongly detected (90.0%). Only 1 of 20 cases PMBCL and 2 of 30 cases of DLBCL were positive for EBER in-situ hybridization.
CONCLUSIONSThe detection rate of immunoglobulin gene rearrangement is significantly lower in PMBCL than that of non-mediastinal DLBCL. However, EB virus infection rates are very low in both types of lymphomas.
