Down-regulation of histone deacetylase 2 induces cell apoptosis and inhibits cell proliferation and migration of laryngeal squamous cell carcinoma cells.
- Author:
Lin-lin YUAN
1
;
Wei-hua LOU
;
Jian-zhong SANG
;
Sheng-lei LI
;
Wen-cai LI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Squamous Cell; metabolism; pathology; Caspase 3; metabolism; Caspase 9; metabolism; Cell Line, Tumor; Cell Movement; Cell Proliferation; Down-Regulation; Histone Deacetylase 2; genetics; metabolism; Humans; Laryngeal Neoplasms; metabolism; pathology; Matrix Metalloproteinase 2; metabolism; Matrix Metalloproteinase 9; metabolism; RNA Interference; RNA, Small Interfering; genetics; Transfection
- From: Chinese Journal of Pathology 2012;41(6):396-399
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of histone deacetylase 2 (HDAC2) expression on cell proliferation, apoptosis and migration of laryngeal squamous cell carcinoma (LSCC) Hep-2 cells.
METHODSHDAC2 siRNA and control siRNA were transfected into LSCC Hep-2 cells by lipofectamine 2000, and cells were divided into three experimental groups: untreated group, control siRNA group and HDAC2 siRNA transfection group. Western blotting was utilized to detect the expression of HDAC2 protein in Hep-2 cells. Cell proliferation and apoptosis were investigated by CCK-8 kit and flow cytometry, respectively. Boyden chamber was used to study cell migration. Expressions of cell apoptosis and cell migration related proteins were detected by Western blotting.
RESULTSHDAC2 siRNA significantly down-regulated the expression of HDAC2 protein in LSCC Hep-2 cells. Down-regulation of HDAC2 expression coincided with an inhibition of cell proliferation and migration along with an induced cell apoptosis of Hep-2 cells. Moreover, down-regulation of HDAC2 expression significantly increased the expressions of caspase-3 and caspase-9 proteins but decreased the expressions of matrix metalloproteinases (MMP)-2 and MMP-9 proteins.
CONCLUSIONSHDAC2 may play a pivotal role in the initiation and development of LSCC. Down-regulation of HDAC2 expression mediates cell apoptosis. Cell migration inhibition may be tightly associated with overexpression of caspase-3 and caspase-9 along with down-regulation of MMP-2 and MMP-9 expressions.
