Reconstruction of dentin-pulp complex structure by tissue engineering technology.
- Author:
Hong-yan GUO
1
;
Bu-ling WU
;
Xi-min GUO
;
Cheng YANG
;
Peng XU
;
Chang-yong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium Phosphates; chemistry; Cells, Cultured; Dental Pulp; cytology; Dentin; cytology; Female; Hydroxyapatites; chemistry; Mice; Mice, Inbred BALB C; Mice, Nude; Rats; Stem Cells; cytology; Tissue Engineering; methods; Tissue Scaffolds; chemistry
- From: Chinese Journal of Stomatology 2005;40(6):511-514
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the possibility of reconstruction of dentin-pulp complex by tissue engineering technology.
METHODSRat dental pulp stem cells were seeded into HA-TCP scaffold and incubated for 20 hours in vitro. Then the cell-scaffold complex was implanted subcutaneously into the dorsal side of nude mice. 8 weeks postimplantation, the samples were extracted for histological and immunohistochemical examinations.
RESULTSThree strata of tissue were observed in the hole of HA-TCP scaffold. They were dentin-like tissue, predentin-like tissue and pulp-like tissue respectively from the inner surface of the pore to the center. Dentin tubules were obvious in predentin-like and dentin-like tissue lining from the pulp-like tissue through predentin-like tissue and dentin-like tissue. Cells localized along the edge of pulp-like tissue were dense and polarized, resembling odontoblasts. Immunohistochemical study demonstrated DSP and DMP1 expression in these odontoblast-like cells and in the area of predentin-like tissue.
CONCLUSIONSTissue-engineered rat dentin-pulp complex was reconstructed by seeding HA-TCP scaffold with rat dental pulp stem cells.
