Inhibitory effect of p53 gene on telomerase activity and proliferative activity in salivary adenoid cystic carcinoma cells.
- Author:
Bing-zhi YAN
1
;
Jie WANG
;
Bo ZHANG
;
Fu-sheng DONG
;
Lin HOU
;
Xu WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Adenoid Cystic; genetics; metabolism; pathology; Cell Line, Tumor; Genetic Vectors; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Salivary Gland Neoplasms; genetics; metabolism; pathology; Telomerase; genetics; metabolism; Transfection; Tumor Suppressor Protein p53; genetics
- From: Chinese Journal of Stomatology 2006;41(1):45-48
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the inhibitory effect of p53 gene on salivary adenoid cystic carcinoma (SACC) cells.
METHODSAdenoviral vector pDeltaE1-p53 was constructed and transfected into SACC-83 cells. The enhanced p53 expression was measured by reverse transcription polymerase chain reaction (RT-PCR), and the effects of transfected p53 on SACC-83 cells were analyzed by TRAP-PCR-ELISA, luciferase reporter, flow cytometry (FCM), soft agar assay and tumorigenicity test.
RESULTSThe expression of p53 gene in SACC-83 cells was increased after introduction of pDeltaE1-p53. The telomerase activity and the transcriptional activity of hTERT promoter were inhibited. The cells cycles of transfected SACC-83 were arrested in G(1) phase and the rate of colony-formation was decreased, and similarly the tumorigenicity in nude mice was also decreased.
CONCLUSIONSThe introduction of wild-type p53 by adenoviral vector could suppress the telomerase activity and malignant phenotypes of salivary adenoid cystic carcinoma cells.
