Effect of fibrinogen on the adherence of Porphyromonas gingivalis to human oral epithelial cells.
- Author:
Song GE
1
;
Ya-fei WU
;
Tian-jia LIU
;
Lei ZHAO
;
Xiao-lu FEI
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Adhesion; drug effects; Fibrinogen; administration & dosage; pharmacology; Humans; KB Cells; Mouth Mucosa; drug effects; microbiology; Periodontitis; etiology; Porphyromonas gingivalis; pathogenicity
- From: Chinese Journal of Stomatology 2006;41(2):69-73
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role of fibrinogen molecule in the pathogenesis of periodontal diseases.
METHODSAn in vitro cell culture model was used. Methyl-(3)H Thymidine radiolabeled Porphyromonas gingivalis (Pg) ATCC 33277 were examined for their ability to adhere to and invade the confluent monolayers of human oral epithelial KB cells with or without exogenous human fibrinogens by scintillation spectrometry.
RESULTSThe addition of exogenous fibrinogens made more amount of and higher ratios of adhesive and invasive Pg, in contrast to the group without exogenous fibrinogen (P < 0.001). At different concentrations of exogenous fibrinogen, the amount and ratios of adhesive and invasive Pg varied significantly (P < or = 0.007). The higher concentrations of exogenous fibrinogen was added, the greater amount and ratios of adhesive and invasive Pg were found.
CONCLUSIONSFibrinogen promotes the adherence of Pg to human oral epithelial cells and may play an important role in the pathogenesis of periodontal diseases.
