Repair of canine segmental mandibular defects using autogenous bone marrow stromal cells and coralline hydroxyapatite.
- Author:
Jie YUAN
1
;
Lian ZHU
;
Min WANG
;
Lei CUI
;
Wei LIU
;
Yi-lin CAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; Bone Substitutes; Ceramics; therapeutic use; Dogs; Hydroxyapatites; therapeutic use; Mandible; physiology; Mandibular Injuries; pathology; surgery; Mesenchymal Stromal Cells; cytology; Tissue Engineering; Tissue Scaffolds
- From: Chinese Journal of Stomatology 2006;41(2):94-97
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo repair segmental mandibular defects with autogenous bone marrow stromal cells (BMSCs) and coralline hydroxyapatite.
METHODSIsolated BMSCs were in vitro expanded and osteogenically induced. In 11 canines, a 3 cm segmental mandibular defect in right mandible was created. Five canine's defects were repaired with cell-scaffold constructs made from induced BMSCs and coralline hydroxyapatite (CHA); Others were repaired with CHA as control. The engineered bone was evaluated by X-ray, CT, gross and histological examination, biomechanical test 12, 26, 32 weeks post-operation respectively.
RESULTSBMSCs grew well on the CHA. X-ray and CT images showed better callus formation at connection sites in experimental group over time while worse formation at connection sites eventually in control group. At 32 weeks post-operation in experimental group, the defects were well repaired grossly. Histologically, there were bony healing and lamellar bone formation, in experimental group fibrous healing and woven bone formation in control group. Biomechanical test revealed no significant difference between experimental group and normal control group.
CONCLUSIONSCanine segmental mandibular defects can be ultimately repaired with the tissue-engineered bone generated by autogenous osteogenic BMSCs and CHA scaffold.
