Effect of Slit-Robo signal on apoptosis of oral cancer cell line Tb.
- Author:
Yu-guang MA
1
;
Li-jing WANG
;
Bing HAN
;
Jie ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Carcinoma, Squamous Cell; metabolism; pathology; Cell Line, Tumor; Fas Ligand Protein; metabolism; Humans; Mouth Neoplasms; metabolism; pathology; Nerve Tissue Proteins; pharmacology; Receptors, Immunologic; Signal Transduction; fas Receptor; metabolism
- From: Chinese Journal of Stomatology 2006;41(4):232-235
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Slit-Robo signal on apoptosis of human oral squamous cell carcinoma line Tb.
METHODSAfter the treatment in Tb cells with monoclonal antibodies (mAb) R5 of against Robo1 receptor extracellular domain, the apoptosis of Tb cell was examined by clone formation assay, flow cytometry, DNA ladder and Hochst-PI. The expression of fas and fasL proteins was observed by Western blotting analysis.
RESULTSAfter the treatment by R5 mAb, the proliferative rate decreased and the apoptotic rates increased, and the expression of fas and fasL proteins was up-regulated.
CONCLUSIONSSlit-Robo signal could inhibit the apoptosis of tumor cells during genesis of tongue cancer by regulating the expression of fas-fasL proteins.
