Effects of As2O3 and all-trans retinoic acid on the growth of HeLa cell line and their relation with gene NDRG1.
- Author:
Xiao-Xing GENG
1
;
Li-Na QUAN
;
Rong MA
;
Li-Ping TANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; administration & dosage; pharmacology; Arsenicals; administration & dosage; pharmacology; Blotting, Western; Cell Cycle Proteins; genetics; metabolism; Cell Proliferation; drug effects; Dose-Response Relationship, Drug; HeLa Cells; Humans; Intracellular Signaling Peptides and Proteins; genetics; metabolism; Oxides; administration & dosage; pharmacology; RNA, Messenger; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Tretinoin; administration & dosage; pharmacology
- From: Chinese Journal of Oncology 2011;33(1):8-12
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of arsenic trioxide (As2O3) and all-trans retinoic acid (ATRA) on human cervical carcinoma HeLa cell line.
METHODSHeLa cells were treated with As2O3 and ATRA. The cell proliferation was evaluated by MTT assay. The expressions of NDRG-1 protein and mRNA were determined by Western blot and RT-PCR analysis.
RESULTSMTT assay showed that As2O3 and ATRA inhibited the growth of human cervical carcinoma HeLa cells in vitro in a dose- and time-dependent manner. Western blot and RT-PCR techniques showed that As2O3 and ATRA down-regulated the expressions of NDRG-1 protein and mRNA (P < 0.05).
CONCLUSIONAs2O3 and ATRA can significantly inhibit the growth and proliferation of HeLa cells. The reason of these changes may be related with the down-regulation of expression of NDRG-1.
